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. 2014 Jan 29;9(1):e86724. doi: 10.1371/journal.pone.0086724

Figure 7. TGR5 did not contributed to the anti-steatosis role of UA in HL-7702 cells.

Figure 7

Cells were seeded into 6-well plate and transfected with siRNA for TGR5 according to the instructions as described in the Methods. Cells were incubated with oleic acid (OA, 0.5 mM) for 24 h, and then OA was removed and the cells were treated with UA (100 µM) for another 24 h. Equal amount of DMSO was added was added into the medium for each group. (A) mRNA level of TGR5 was detected for the analysis of transfection efficiency. (B) The intracellular TG was detected by triglyceride assay kit (Applygen Technologies Inc, Beijing, China) according to the manufacturer’s instructions. Different alphabet indicates statistically significant differences (P<0.05). Data were expressed as means ± SD. All the experiments were repeated at least 3 times.