Skip to main content
. 2014 Jan 29;9(1):e86724. doi: 10.1371/journal.pone.0086724

Table 2. Primer sequence for quantitative real-time PCR.

Gene Sequence
β-actin F: 5′-AGGGAAATCGTGCGTGAC-3′
R: 5′-CGCTCATTGCCGATAGTG-3′
PPAR-α F: 5′-TCATACTCGCAGGAAAGACT-3′
R: 5′-ACCTCTGCCTCCTTGTTTTC-3
FAT/CD36 F: 5′-CGGCGATGAGAAAGCAGAA-3′
R: 5′-CCAGGCCCAGGAGCT TTATT-3′
DGAT1 F: 5′-TTTCTGCTACGGCGGGTTCTTGAG-3′
R: 5′-ACCGGTTGCCCAATGATGAGTGTC-3′
DGAT2 F: 5′-GGAGGCCACCGAAGTTAGCAAGAA-3'
R: 5′-AGCCCCCAGGTGTCAGAGGAGAAG-3′
ATGL F: 5′-CACTTTAGCTCCAAGGATGA-3′
R: 5′-TGGTTCAGTAGGCCATTCCT-3′
HSL F: 5′-ACATGGCCTTCTTCTCAAGC-3′
R: 5′-TCATGGGATTTGGAGGTCTG-3′
CPT-1 F: 5′-GCCCATGTTGTACAGCTTCCA-3′
R: 5′-AGTCTTCTTCCTTCATCAGTGGC-3′
SREBP-1c F: 5′-GCCATCGACTACATCCGCTT-3′
R: 5′-CAGGTCTTTCAGTGATTTGCTTTT-3′
FAS F: 5′-TCTCTGGTGGTGTCTACATTTCG-3′
R: 5′-GCAGGATAGCACTCTCAGACAG-3′
ACC F: 5′-TTTTCGATGTCCTCCCAAACTTTT-3′
R: 5′-GCTCATAGGCGATATAAGCTCTTC-3′
TNF-α F: 5′-TGTCTCAGCCTCTTCTCATT-3′
R: 5′-AGATGATCTGAGTGTGAGGG-3′
CCL2/MCP-1 F: 5′-CTGTCTCAGCCAGATGCAGTTAA-3′
R: 5′-AGCCGACTCATTGGGATCAT-3′
IL-1β F: 5′-CTGGTACATCAGCACCTCTCAA-3′
R: 5′-GAGACTGCCCATTCTCGACAA-3′
IL-2 F: 5′-ACAAGAATCTGAAACTCCCC-3′
R: 5′-GAGATGATGCTTTGACAGATGG-3′
IL-6 F: 5′-GCCACTGCCTTCCCTACTTCA-3′
R: 5′-GACAGTGCATCATCGCTGTTCA-3′
IL-8 F: 5′-CATTAATATTTAACGATGTGGATGCGTTTCA-3′
R: 5′-GCCTACCATCTTTAAACTGCACAAT-3′
PPAR-α(h) F: 5′-TCGACTCAAGCTGGTG-3′
R: 5′-TTCCTGAGAGGATGACCC-3′
CPT-1 (h) F: 5′-GCCCATGTTGTACAGCTTCCA-3′
R: 5′-AGTCTTCTTCCTTCATCAGTGGC-3′
FXR (h) F: 5′- TGGACTCATACAGCAAACAGAGA -3′
R: 5′- GTCTGAAACCCTGGAAGTCTTTT -3′
LXR F: 5′-TCAGCATCTTCTCTGCAGACCGG-3′
R: 5′- TCATTAGCATCCGTGGGAACA -3′
TGR5 F: 5′- CTGGCCCTGGCAAGCCTCAT -3′
R: 5′- CTGCCATGTAGCGCTCCCCGT -3′
β-actin (h) F: 5′-ACTATCGGCAATGAGCG-3′
R: 5′-GAGCCAGGGCAGTAATCT-3′

(h) indicated that the primer is used for the detection of human gene.