Table 2. Primer sequence for quantitative real-time PCR.
Gene | Sequence |
β-actin | F: 5′-AGGGAAATCGTGCGTGAC-3′ |
R: 5′-CGCTCATTGCCGATAGTG-3′ | |
PPAR-α | F: 5′-TCATACTCGCAGGAAAGACT-3′ |
R: 5′-ACCTCTGCCTCCTTGTTTTC-3 | |
FAT/CD36 | F: 5′-CGGCGATGAGAAAGCAGAA-3′ |
R: 5′-CCAGGCCCAGGAGCT TTATT-3′ | |
DGAT1 | F: 5′-TTTCTGCTACGGCGGGTTCTTGAG-3′ |
R: 5′-ACCGGTTGCCCAATGATGAGTGTC-3′ | |
DGAT2 | F: 5′-GGAGGCCACCGAAGTTAGCAAGAA-3' |
R: 5′-AGCCCCCAGGTGTCAGAGGAGAAG-3′ | |
ATGL | F: 5′-CACTTTAGCTCCAAGGATGA-3′ |
R: 5′-TGGTTCAGTAGGCCATTCCT-3′ | |
HSL | F: 5′-ACATGGCCTTCTTCTCAAGC-3′ |
R: 5′-TCATGGGATTTGGAGGTCTG-3′ | |
CPT-1 | F: 5′-GCCCATGTTGTACAGCTTCCA-3′ |
R: 5′-AGTCTTCTTCCTTCATCAGTGGC-3′ | |
SREBP-1c | F: 5′-GCCATCGACTACATCCGCTT-3′ |
R: 5′-CAGGTCTTTCAGTGATTTGCTTTT-3′ | |
FAS | F: 5′-TCTCTGGTGGTGTCTACATTTCG-3′ |
R: 5′-GCAGGATAGCACTCTCAGACAG-3′ | |
ACC | F: 5′-TTTTCGATGTCCTCCCAAACTTTT-3′ |
R: 5′-GCTCATAGGCGATATAAGCTCTTC-3′ | |
TNF-α | F: 5′-TGTCTCAGCCTCTTCTCATT-3′ |
R: 5′-AGATGATCTGAGTGTGAGGG-3′ | |
CCL2/MCP-1 | F: 5′-CTGTCTCAGCCAGATGCAGTTAA-3′ |
R: 5′-AGCCGACTCATTGGGATCAT-3′ | |
IL-1β | F: 5′-CTGGTACATCAGCACCTCTCAA-3′ |
R: 5′-GAGACTGCCCATTCTCGACAA-3′ | |
IL-2 | F: 5′-ACAAGAATCTGAAACTCCCC-3′ |
R: 5′-GAGATGATGCTTTGACAGATGG-3′ | |
IL-6 | F: 5′-GCCACTGCCTTCCCTACTTCA-3′ |
R: 5′-GACAGTGCATCATCGCTGTTCA-3′ | |
IL-8 | F: 5′-CATTAATATTTAACGATGTGGATGCGTTTCA-3′ |
R: 5′-GCCTACCATCTTTAAACTGCACAAT-3′ | |
PPAR-α(h) | F: 5′-TCGACTCAAGCTGGTG-3′ |
R: 5′-TTCCTGAGAGGATGACCC-3′ | |
CPT-1 (h) | F: 5′-GCCCATGTTGTACAGCTTCCA-3′ |
R: 5′-AGTCTTCTTCCTTCATCAGTGGC-3′ | |
FXR (h) | F: 5′- TGGACTCATACAGCAAACAGAGA -3′ |
R: 5′- GTCTGAAACCCTGGAAGTCTTTT -3′ | |
LXR | F: 5′-TCAGCATCTTCTCTGCAGACCGG-3′ |
R: 5′- TCATTAGCATCCGTGGGAACA -3′ | |
TGR5 | F: 5′- CTGGCCCTGGCAAGCCTCAT -3′ |
R: 5′- CTGCCATGTAGCGCTCCCCGT -3′ | |
β-actin (h) | F: 5′-ACTATCGGCAATGAGCG-3′ |
R: 5′-GAGCCAGGGCAGTAATCT-3′ |
(h) indicated that the primer is used for the detection of human gene.