Figure 3. Microbubbles targeted with anti-chicken IgY were retained within angiosarcoma vasculature at significantly lower levels than microbubbles loaded with streptavidin.

Streptavidin-coated microbubbles were bound to a mixture of biotinylated anti-chicken IgY (raised in rabbit and goat) to produce anti-chicken IgY control microbubbles. Three-dimensional molecular imaging with the anti-chicken IgY control microbubbles resulted in significantly lower average pixel intensity (*p = 0.0002, n = 10, unpaired t-test, two-tailed) than observed with microbubbles coated only with streptavidin.