Table 1. Genes encoding enzymes of the JH biosynthetic pathway in Apis mellifera. Prediction based on BLASTP comparisons using Anopheles gambiae and Drosophila melanogaster sequences against the honey bee genome (version 4.0).
| Enzyme | Symbol | Function | E.C. number | A. gambiae ortholog | D. melanogaster ortholog | A. mellifera ortholog | E-value |
| Methionine adenosyl transferase | MAT | Synthesis of S-adenosyl-L-methionine (AdoMet) | 2.5.1.6 | XM_307861 | NM_164362 | XM_623666 | e-132 |
| Adenosylhomocysteinase | AHC1 | Hydrolysis of S-adenosyl-L-homocysteine (AdoHcy) | 3.3.1.1 | XM_311257 | NM_078609 | XM_391917 | 0.0 |
| AHC2 | XM_624149 | e-124 | |||||
| Adenosine kinase | AK | Phosphorylates adenosine | 2.7.1.20 | XM_307001 | NM_168532 | XM_391988 | e-110 |
| Acetoacetyl CoA thiolase | ACT | Condenses 2 molecules of Acetyl-CoA | 2.3.1.9 | XP_321828 | NP_612094 | XM_391843 | 2e-77 |
| Hydroxymethylglutaryl-CoA redutase | HMGR | Reduces HMG-CoA to mevalonate | 1.1.1.34 | XP_307890 | - | XM_623115 | 0.0 |
| Hydroxymethylglutaryl-CoA synthase | HMGS | Condenses Acetoacetyl-CoA+acetyl-CoA | 2.3.3.10 | XP_315872 | NP_725570 | XM_397202 | e-171 |
| Mevalonate kinase | MK | Phosphorylates mevalonate | 2.7.1.36 | XM_319701 | NM_176158 | XM_624542 | 1e-30 |
| Phosphomevalonate kinase | PMK | Phosphorylates phosphomevalonate | 2.7.4.2 | XP_310779 | Q9VIT2 | XM_001120920 | 1e-34 |
| Isopentenyl-diphosphate delta-isomerase | IPPI | Isomerization of IPP to DMAPP | 5.3.3.2 | XP_321388 | NP_650962 | XM_001121223 | 4e-27 |
| Farnesyl diphosphate synthase (prenyltransferase) | FPPS1 | Sequential condensation of IPP with DMAPP and then GPP to form FPP | 2.5.1.1/10 | XP_308653 | NP_477380 | XM_001122575 | 2e-66 |
| FPPS2 | XM_623583 | 8e-79 | |||||
| FPPS3 | XM_326224 | 8e-76 | |||||
| FPPS4 | XM_001122685 | 5e-59 | |||||
| FPPS5 | XM_624295 | 2e-59 | |||||
| FPPS6 | XM_001123325 | 1e-37 | |||||
| Citrate (si)-synthase | CS | Synthesis of citrate in the mitochondria | 2.3.3.1 | XM_320478 | - | XM_393545 | 0.0 |
| ATP citrate lyase | ATPCL | Synthesis of cytosolic acetyl-CoA from citrate | 2.3.3.8 | XM_319323 | - | XM_623080 | 0.0 |
| Mitochondrial citrate transport protein | MCTP1 | Transports citrate from mitochondria to cytosol | - | XP_308964 | NP_727450 | NM_001010975 | e-128 |
| MCTP2 | XM_395934 | e-110 | |||||
| Short-chain dehydrogenase | SCD | Oxidation of farnesol to farnesal? | - | XM_556135 | NM_132467 | XM_624031 | 5e-57 |
| Crustacean “Farnesoic acid O-methyltransferase” homolog | FAMET | Function unclear | - | XP_318631 | NP_611544 | XM_623143 | e-101 |
| Bombyx JHA methyl transferase ortholog | MT | Transfers methyl group from AdoMet to farnesoic acid | - | XP_314173 | NP_609793 | XM_001119986 | 1e-40 |
| Methyl farnesoate epoxidase (CYP15) | MFE | Oxidation of MF into JH III | - | XP_315675 | NP_649151 | XM_623572 | 5e-80 |
Enzyme Commission (E.C.) classification and enzyme functions are shown.