Table 2. Retention time (Rt), molecular weight (MW), prevalence, and mean relative area of each peptide based on HPLC-MS chromatograms.
Rt (min) | MW | Prevalence (%) | Mean relative area |
Colostethus panamansis (Nā=ā36) | |||
3.6 | 1041.5 | 61 | 0.14 |
4.45 | 1064.0 | 100 | 1.00 |
4.5 | 3306.6 | 72 | 0.35 |
4.8 | 1936.2 | 25 | 0.12 |
5.0 | 1512.0 | 42 | 0.28 |
5.3 | 2988.6 | 3 | 0.03 |
5.3 | 3001.5 | 3 | 0.02 |
5.7 | 2986.6 | 6 | 0.20 |
7.0 | 2974.5 | 17 | 0.65 |
6.8 | 2957.7 | 17 | 0.57 |
7.1 | 2972.6 | 17 | 0.93 |
7.0 | 2290.4 | 11 | 1.07 |
7.3 | 2231.4 | 6 | 0.63 |
7.6 | 2315.4 | 6 | 0.94 |
8.1 | 1005.6 | 94 | 1.66 |
9.7 | 1790.0 | 6 | 0.13 |
Espadarana prosoblepon (Nā=ā16) | |||
1.45 | 1746.7 | 81 | 0.02 |
2.2 | 1585.8 | 94 | 0.31 |
3.7 | 2650.2 | 100 | 0.72 |
4.4 | 2698.2 | 100 | 1.14 |
4.55 | 2634.2 | 100 | 0.74 |
4.6 | 2652.2 | 100 | 0.37 |
4.75 | 1004.6 | 100 | 0.39 |
5.05 | 1004.6 | 100 | 0.51 |
5.45 | 2681.2 | 100 | 1.00 |
5.69 | 2665.1 | 38 | 0.06 |
Area is relative to a consistently observed peak, MW 1064.0 for C. panamansis and MW 2681.2 for E. prosoblepon.