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. 2014 Jan 29;9(1):e87098. doi: 10.1371/journal.pone.0087098

Figure 4. Infection with rRABV results in the upregulation of B cell activation markers, which are enhanced by infection with rRABV-mICAM-1.

Figure 4

The infected splenocyte cultures described in Figure 3 were analyzed for the activation of B cells, as measured by the upregulation of cell surface expression of CD69, CD40 or MHCII in RABV N+ B cells. Representative gating strategy of B220+ B cells from the total live population gated on CD69 (A), CD40 (C) or MHCII (E). The percentage of RABV N+CD69+ B cells (B), RABV N+CD40+ B cells (D) and RABV N+MHCII+ B cells (F) are indicated. G) Pretreatment of sucrose-purified rRABV-mICAM-1 with a neutralizing anti-ICAM-1 antibody significantly reduced the expression of MHCII on the infected B cells in culture. To compare two groups of data, we used an unpaired, two-tailed Student's t test. (* p<0.05; **, p<0.01; ***, p<0.001)