. Author manuscript; available in PMC: 2014 Dec 23.

Published in final edited form as: Biochemistry. 2013 Dec 13;52(51):9223–9236. doi: 10.1021/bi400987k

Table 5.

Hydrodynamic parameters for WW domains alone and in the context of WW1–WW2 tandem module of WWOX

Construct	M_w/kD	M_n/kD	M_w/M_n	R_h/Å	Associativity
WW1	9 ± 2	9 ± 1	1.06 ± 0.08	18 ± 1	Monomer
WW2	10 ± 1	9 ± 1	1.02 ± 0.03	19 ± 1	Monomer
WW1–WW2	14 ± 1	14 ± 1	1.03 ± 0.04	27 ± 1	Monomer

All parameters were obtained from ALS measurements at pH 7.0 and 25°C. Note that the calculated molar masses of recombinant WW1, WW2 and WW1–WW2 constructs from their respective amino acid sequence are 9kD, 9kD and 14kD, respectively. Errors were calculated from at least three independent measurements. All errors are given to one standard deviation.