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. 2014 Jan 29;9(1):e87194. doi: 10.1371/journal.pone.0087194

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© 2014 Dacheux et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic representation of the VP1 segment (almost 3,300 nt encoding the RNA-dependent RNA polymerase of almost 1,090 aa) of the genome of the lamb rotavirus strain Lamb-NT (GenBank number FJ031024), with black bars corresponding to the longest contig sequences (>300 nt) of the bat rotavirus (named Maule rotavirus) identified in specimen b8 (Myotis mystacinus). The genomic region amplified by PCR is represented by a dashed bar, and the sequence used for phylogenetic analysis is indicated with an asterisk. B) Phylogenetic tree produced from the amino-acid alignment based on the partial VP1 sequence (119 aa, positions 964 to 1082 of the VP1 protein of lamb rotavirus strain Lamb-NT) translated from one of the longest HSPs. The bat rotavirus-related sequence is indicated in bold within the various rotavirus groups. The scale bar indicates branch length, and bootstrap values ≥70% are shown next to the relevant nodes. The tree is midpoint-rooted for purposes of clarity only.