Table 3. Identification and distribution of sequences of vertebrate viruses of interest among the various bat specimens and tissue samples analyzed.
Bat species, specimens and tissue samplesa | ||||||||||||||||||||||||||
Pipistrellus pipistrellus | Hypsugo savii | Myotis nattereri | Eptesicus serotinus | Myotis mystacinus | ||||||||||||||||||||||
b1 | b2 | b3 | b4 | b9 | b5 | b6 | b7 | b8 | ||||||||||||||||||
Virus | Sequence detectionb | Br | Li | Lu | Br | Li | Lu | Br | Li | Lu | Br | Li | Lu | Li | Lu | Br | Li | Lu | Br | Li | Lu | Br | Li | Lu | Li | Lu |
Nairovirus | HTS | −c (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | +c (tissue pool) [S, M, L] | − (tissue pool) | − (tissue pool) | − (tissue pool) | + (tissue pool) [S, M, L] | ||||||||||||||||
PCR/Sanger | − | − | − | − | − | − | − | − | − | − | − | − | − | + [L] | − | − | − | − | − | − | − | − | − | − | + [L] | |
Rotavirus | HTS | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | + (tissue pool) [VP1–2, VP4, VP7] | ||||||||||||||||
PCR/Sanger | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | + [VP1–2] | |
Gammaretrovirus | HTS | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | + (tissue pool) [env, pol] | − (tissue pool) | ||||||||||||||||
PCR/Sanger | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | - | + [pol] | − | − | |
Bornavirus | HTS | + (tissue pool) [L] | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | + (tissue pool) [L] | − (tissue pool) | − (tissue pool) | ||||||||||||||||
PCR/Sanger | + [L] | − | − | + [L] | − | − | + [L] d | − | + [L] | + [L] | − | − | + [L] d | − | + [L] d | − | + [L] | + [L] | − | + [L] d | − | − | − | − | − | |
Adenovirus | HTS | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | − (tissue pool) | + (tissue pool) [multiple genes] | − (tissue pool) | − (tissue pool) | ||||||||||||||||
PCR/Sanger | NDe | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
Picobirnavirus | HTS | + (tissue pool) [RdRp] | − (tissue pool) | − (tissue pool) | + (tissue pool) [RdRp] | + (tissue pool) [RdRp] | + (tissue pool) [RdRp] | − (tissue pool) | − (tissue pool) | + (tissue pool) [RdRp] | ||||||||||||||||
PCR/Sanger | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
Legend :
Br = brain, Li = liver, Lu = lungs.
Matching (HTS) or targeting (PCR/Sanger) viral genes (in brackets), with S = small, M = medium, L = large or polymerase, RdRp = RNA-dependent RNA polymerase.
+ = positive, - = negative.
PCR products larger than expected.
ND = not done.