Skip to main content
. 2014 Jan 29;9(1):e87086. doi: 10.1371/journal.pone.0087086

Figure 7. Expression of the myeloid marker CD33 and the effect of blocking CD33 on the differentiation of NK cells from CBCD34+ and PBCD34+.

Figure 7

(A) Percentage of CD45+CD33+ cells at different time points for CBCD34+ (n = 8) and PBCD34+ (n = 6) cultures. (B) FACS plots showing CD33 expression against CD56 at different time points for CBCD34+ (n = 8) and PBCD34+ (n = 6) cultures. NK cells from CBCD34+ (n = 4–6) or PBCD34+ (n = 3–5) were incubated in the presence or not of a CD33 blocking antibody. Secreted IFN-γ (C), CD107a degranulation (D), intracellular IFN-γ (E) and NK cell killing in vitro of the malignant cell line K562 in a 10∶1 effector-target ratio (F) were evaluated. Statistical analysis was performed using paired T-test. * P<0.05.