Figure 1. Subcellular Localization of APUM5-GFP in N. benthamiana. (A) C-terminal GFP-tagged APUM5 was transiently expressed in N. benthamiana. The FM4-64 stain was used as a plasma membrane marker. Merged image shows colocalized regions. Scale bars are indicated at the pictures. (B) APUM5-GFP signals were merged with FM4-64 in the plasma membrane. (C) APUM5-GFP signals were merged with FM4-64 in vacuolar-like membrane. (Band C) Images are enlarged images of those indicated as boxes and circles in the (A) image. (D) APUM5-GFP was localized to vesicle-like structures and nucleus. The arrow indicates the nucleus. Red signals are chloroplast-auto signals. Scale bars are indicated at the pictures. (EandF) Enlarged images of those indicated by boxes in (D).