Table 1. Change in secretory vesicle instant speed in the subcortical conveyor and the cortical exchange zones.

		Caged		Free		Directed
Type of motion	Stimulation	Instant speed(µm/s, ×10−2)	n	Instant speed(µm/s, ×10−2)	n	Instant speed(µm/s, ×10−2)	n
Subcortical conveyor zone	Before	3.90±0.12	700	4.33±0.08	1347	4.18±0.10	887
	During	3.70±0.01NS	628	4.14±0.09NS	1123	5.00±0.15***	578
Cortical exchange zone	Before	3.89±0.08	971	4.31±0.06	2082	4.31±0.07	1944
	During	3.30±0.06***	1577	3.91±0.07***	2043	4.37±0.09NS	1172

For all the tracked vesicles, instant speed at all tracking points of the vesicle was recorded with Imaris and sorted depending on the cellular location of the vesicle and its type of motion (subcortical transfer zone is 1.5–2.5 µm from the membrane, and cortical exchange zone is 0–1.5 µm from the membrane). The average instant speed of the vesicles in each given state was then calculated in the two areas. Note the significant change in instant speed of directed vesicles in both areas of the cell. Mean ± S.E.M.

^*p<0.05,

^***p<0.0001, ^NS: not significant.