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. 2014 Jan 29;9(1):e87409. doi: 10.1371/journal.pone.0087409

Figure 4. Snail is crucial for properties attributed to cancer stem cells.

Figure 4

A. Snail silencing decreases ALDHhigh population in Panc-1 cells. Dot plots of cells analyzed by flow cytometry for ALDH activity. The values presented are the averages of three independent experiments. B. Snail silencing significantly inhibits the ability of sphere formation with serial passaging and the capability of the clonogenicity in Panc-1 cells. Representative pictures of colony are shown above the column diagram. Similar experiments were repeated three times. ** P<0.01, compared with Panc-1/NC. C. Western blot analysis of cell extracts from Panc-1/NC and Panc-1/shSnail cells for Bmi1, Nanog, Sox2, and Oct4 expression. GAPDH was used as a loading control. D. Quantification of protein levels of Bmi1, Nanog, Sox2, and Oct4 in Panc-1/NC and Panc-1/shSnail cells. * P<0.05, **P<0.01 compared with Panc-1/NC.