Figure 4. T. cruzi PIGB can complement a yeast strain deficient in both gpi10 and smp3.

A. Schematic of a tetrad showing the desired non-parental ditype pattern of kanamycin marker segregation generated from the mating of a haploid gpi10::Kan^R strain containing pGAL-TcrPIGB with a haploid smp3::Kan^R strain containing pGAL-TcrPIGB. B. PCR was used to test each haploid meiotic segregant from a non-parental ditype tetrad for the presence of gpi10Δ and smp3Δ alleles (see Figure 3B for PCR schematic). Haploids 1 and 2 are each double mutants that contain both null alleles. C. Serial drop cultures of both single gpi10Δ and smp3Δ mutant strains as well as the double mutant gpi10Δ/smp3Δ strain. Note that the strains grow on the galactose-containing medium that induces pGAL-TcrPIGB expression but not on the glucose medium, which represses pGAL-TcrPIGB expression.