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. 2014 Jan 29;9(1):e87673. doi: 10.1371/journal.pone.0087673

Figure 5. The long and short conserved motifs do not confer broad specificity to TcrPigB.

Figure 5

A. Alignment of the N-terminal long and C-terminal short motifs from yeast and protozoa. B. Schematics of the generated chimera constructs. The N-terminal long (chimera 1), C-terminal short (chimera 2) or both conserved motifs (chimera 3) of TcrPIGB (red) were replaced by those from TbPIGB (green). Numbers correspond to the amino acid positions of each domain within its native protein sequence. C. Serial drop cultures of control and chimeric constructs in both gpi10Δ and smp3Δ strains before (SGal) and after (SD + 5FOA) plasmid shuffling. Note that all three of the chimeric PigB proteins are able to complement the gpi10Δ and smp3Δ mutant strains.