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. 2014 Jan 29;9(1):e87668. doi: 10.1371/journal.pone.0087668

Figure 1. IL-4 suppresses the expression of TLR7- and 9-induced IFN-responsive gene expression.

Figure 1

We treated cDCs at day 6-7 of culture with IL-4 or left them untreated for 24 h. We then stimulated the cDCs with CpG 1826 (10 ug/ml) or R848 (1 ug/ml) for 6 h and then analyzed by qPCR the expression of the IFN-responsive genes ISG15 and Mx-1. All of the conditions were normalized against the control (untreated DCs in medium only) in each experiment. Results are average of three independent experiments, performed with three independent bone marrow-derived cultures from 3 different mice; *p<0.05; **p<0.01.