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. 2014 Jan 29;9(1):e87668. doi: 10.1371/journal.pone.0087668

Figure 7. IL-4 suppresses TLR7 and TLR9 at the gene but not the protein level in cDCs.

Figure 7

A. We analyzed by qPCR the gene expression of TLR7 and TLR9 in cDCs treated or not with IL-4 for 24 h. Graphs show averages and SE of six independent cultures. B. We analyzed the protein expression of TLR7 in the total lysates of cDC treated or not for 24 h with IL-4. C. We analyzed by immunofluorescence staining TLR7 expression in cDCs after 24 h treatment with IL-4. The nucleus was stained with DAPI and TLR7 was stained using a rabbit polyclonal anti-body to TLR7 and a secondary anti-rabbit IgG conjugated to Cy3. A minimum of five fields in each slide was read for each condition at a magnification of 40×. Picture representative of five independent cultures.