Foxq1 promotes cell invasion and migration in vitro. A and B, the ectopic expression of Foxq1 (left), migration (middle), and invasion (right) assay shows that the ectopic expression of Foxq1 leads to an increase in cel migration and an increase in cell invasion in HMLE cells (A) and in EpRas cells (B). C, the shRNA knockdown of Foxq1 (left), migration (middle), and invasion (right) assay shows that the knockdown of Foxq1expression leads to a decrease in migration (P= 0.002) and invasion ability (P= 0.014) in 4T1 cells. D, the expression of human Foxq1 (left), migration (middle), and invasion (right) assay shows that the expression of hFoxq1 leads to an increase in migration (P= 0.002) and invasion ability (P= 0.014) in 4T1 cells with mFoxq1 knockdown. All experiments were performed in triplicate. The data number was the mean value from 5 randomly selected field views. Each bar represents the mean ± SEM (standard error of the mean).