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. Author manuscript; available in PMC: 2014 Jan 29.
Published in final edited form as: Cancer Res. 2011 Feb 1;71(4):1292–1301. doi: 10.1158/0008-5472.CAN-10-2825

Figure 6.

Figure 6

Transcriptional regulation of E-cadherin by . A, schematic representation of the Luc-reporter construction, showing location of the 3 E-boxes in the promoter region of E-cadherin gene. The black boxes show the mutant form. B, Foxq1 downregulates the E-cadherin promoter in a dose-dependent manner. Samples 1–4, which show variable amounts of the Foxq1 expression construct and pcDNA3.1 vector, were cotransfected into 293FT cells with the E-cadherin promoter plasmid. Bars, SD. C, mutation of the E-boxes impairs repression of the E-cadherin promoter by Foxq1. Data are expressed as Luc activity in the presence of Foxq1 as a percentage of the activity of the same reporter in the presence of the control vector. Bars, SD. D, direct interaction between Foxq1 and the E-cadherin promoter. Anti-V5 coprecipitated chromatin was subjected to E-cadherin promoter-specific PCR amplification. PCR amplification from the total chromatin (Input, lane 2) was used as a positive control and those lacking Foxq1 expression (lane 1) or anti-HA (lane 3) and a pair of primers located in a distant region of E-cadherin promoter (bottom panel) served as diverse negative controls.