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. 2014 Jan 29;9(1):e87785. doi: 10.1371/journal.pone.0087785

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© 2014 Cailotto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panel A shows the site of dextran amine injection at the level of the DMV, 19 days after injection. The tracer was revealed by DAB staining. B. Epifluorescent picture shows the distribution of Texas-red dextran amines after lateral ventricle injection. In the panels C & D, arrow heads show the presence of dextran amines (revealed by phosphatase alkaline staining to amplify the Texas red signal of the tracer) in the deep cervical lymph node. Of note, the presence of the tracer was found in all LV injected mice (D) and occasionally in DMV-injected mice (C). 3 V: third ventricle. LV: lateral ventricle. DMV: dorsal motor nucleus of the vagus. Scale bar represents 0.1 mm.