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. 2014 Jan 29;9(1):e87785. doi: 10.1371/journal.pone.0087785

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© 2014 Cailotto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Epifluorescence images collected for the identification of the neurotransmitters (red) and dextran-labeled vagus efferent fibers and terminals (Green). Choline acetylfransferase (ChAT, A), neuronal nitric oxide synthase (nNOS, B), Tyrosine hydroxylase (TH, C), substance P (SP, D), vasoactive intestinal peptide (VIP, E) and Calcitonin gene related peptide (CGRP, F). Arrow heads point discrete co-localization between Vagus nerve and VIP or CGRP positive structures. Vagus nerve efferent fibers and terminals are only positive for ChAT and located in close proximity to ChAT and nNOS enteric neuronal bodies. Scale bars represent 20 µm.