Figure 5. Reduction of cerebral Aβ pathology in APP/PS1 mice immunized with 4Aβ1-15. (A, B, E and F) The hippocampus. (C, D, G and H) The parietal cortex. (left) PBS-immunized APP/PS1 mice. (right) 4Aβ1-15-immunized APP/PS1 mice. (A–D) Mouse brain coronal sections were stained with monoclonal anti-human Aβ antibody 4G8. (I) Percentages (plaque burden, area plaque/total area) of Aβ antibody-immunoreactive Aβ plaques were calculated by quantitative image analysis and reductions for each mouse brain area analyzed are indicated. (E–H) Mouse brain sections from the indicated regions were stained with thioflavin T. (J) Percentages of thioflavin T-stained plaques were quantified by image analysis, and reductions for each brain region are indicated (n = 9, ^#p < 0.01). (Scale bar: 200 μm.)