Figure 6. 4Aβ1-15 epitope vaccine reduces microglial activation. (A, B, E and F) The hippocampus. (C, D, G and H) The parietal cortex. (left) PBS-immunized APP/PS1 mice. (right) 4Aβ1-15-immunized APP/PS1 mice. Image analysis of cortex areas from vaccinated or control mice were performed after staining with anti-I-A/I-E antibody (red) or anti-CD45 antibody (green). Vaccination with the epitope vaccine decreased microglial activation in hippocampal brain regions of immune mice compared with that in control mice. Representative pictures of brain sections stained with each antibody showing a decreased immunoreactivity also in the cortical region in mice immunized with the epitope vaccine compared with control animals. (I and J) The quantitative image analyses indicated that vaccinated mice had a lesser degree of microglia activation compared with the control group (n = 9, ^#p < 0.01). (Scale bar: 200 μm.)