Figure 1. Humanization of murine antibody 225 to create hu225. heavy (A) and light (B) variable domains of 225, hu225 and human acceptor frameworks 60P2 and NOV. CDRs (Kabat definition) are underlined in 225. Framework positions in hu225 where murine residues were retained are shown in bold and underlined. At VH position 12 double underlined and bold indicates where the infrequent Ile found in 60P2 was changed to Val as found in the human VH3 family consensus.