Table 2. Hu225 pyrosequencing summary.
| FACS gate | type | number | Ave. length | 3 CDRs |
|---|---|---|---|---|
| bp | % | |||
| Binding | VH/F | 105,866 | 310 | 79 |
| “ | VH/R | 83,792 | 315 | 81 |
| “ | VL/F | 97,846 | 311 | 84 |
| “ | VL/R | 108,662 | 309 | 85 |
| Expression | VH/F | 112,431 | 315 | 80 |
| “ | VH/R | 95,051 | 321 | 87 |
| “ | VL/F | 116,288 | 311 | 86 |
| “ | VL/R | 111,096 | 308 | 86 |
| Total | 831,032 |
Distribution of reads between the two FACS gates, heavy (VH) and light (VL) chains, forward (F) and reverse (R) reads. Average read length and the fraction of reads for which all 3 CDRs were identified are indicated.