Table 4. Affinity measurements for selected higher affinity hu225 variants.
| variant | ER for codon(s) | codon rank(s) | ka (M−1s−1) | kd (s−1) | KD (nM) | WT/x |
|---|---|---|---|---|---|---|
| hu225 | 5.4E+05 | 4.6E-03 | 8.5 | 1.0 | ||
| VH:Y32R | 7.22, 6.61, 5.90 | 79, 100, 117 | 5.7E+05 | 3.9E-03 | 6.8 | 1.3 |
| VH:G33D | 8.11 | 54 | 8.3E+05 | 1.6E-03 | 1.9 | 4.5 |
| VH:V50L | 10.87, 6.89. 6.46 | 15, 90, 104 | 5.2E+05 | 1.6E-03 | 3.1 | 2.7 |
| VH:V50Q | 12.73 | 3 | 5.5E+05 | 1.6E-03 | 2.9 | 2.9 |
| VH:I51G | 7.67, 6.88 | 64, 92 | 5.9E+05 | 2.1E-03 | 3.6 | 2.4 |
| VH:T57G | 7.09, 6.60 | 85, 101 | 5.6E+05 | 2.2E-03 | 3.9 | 2.2 |
| VH:T57P | 8.74, 6.56 | 42, 102 | 5.6E+05 | 2.1E-03 | 3.8 | 2.2 |
| VH:T97D | 6.4 | 108 | 7.0E+05 | 1.1E-03 | 1.6 | 5.3 |
| VH:Y98W | 4.82 | 151 | 6.6E+05 | 3.8E-03 | 5.8 | 1.5 |
| VH:F100cY | 14.19 | 1 | 6.3E+05 | 2.5E-03 | 4.0 | 2.1 |
| VL:A25V | 10.70, 9.71 | 17, 29 | 6.0E+05 | 1.8E-03 | 3.0 | 2.8 |
| VL:Q27Y | 10.47 | 21 | 5.6E+05 | 1.2E-03 | 2.1 | 4.0 |
| VL:G30Y | 11.51 | 11 | 6.1E+05 | 1.1E-03 | 1.8 | 4.7 |
| VL:T97C | 14 | 2 | 5.6E+05 | 2.0E-03 | 3.6 | 2.4 |
| VL:T97D | 5.98 | 112 | 6.1E+05 | 1.7E-03 | 2.8 | 3.0 |
| VL:T97S | 12.44, 11.80, 10.80 | 7, 8, 16 | 5.7E+05 | 1.5E-03 | 2.6 | 3.3 |