Table 3. Percent scrambled disulfides at different digestion pHs for rituximab and RNA-mediated mAb.
| pH 8.0 | pH 6.8 | pH 2.0 | ||||
|---|---|---|---|---|---|---|
| Rituximab (%) | RNAi-anti-CD20 (%) | Rituximab (%) | RNAi-anti-CD20 (%) | Rituximab (%) | RNAi-anti-CD20 (%) | |
| T2H(C22)-T20H(C265) | 2.2 | 0.4 | 1.6 | 0.0 | N.D. | N.D. |
| T2H(C22)-T34H(C371) | 0.4 | 0.1 | 0.3 | N.D. | N.D. | N.D. |
| T9H(C96)-T26H(C325) | 0.2 | 0.1 | N.D. | N.D. | N.D. | N.D. |
| T12H(C148)-H26H(C325) | 0.3 | 0.6 | 0.0 | 0.1 | N.D. | N.D. |
| T17H(C224)-T20H(C265) | 0.3 | 0.6 | N.D. | N.D. | N.D. | N.D. |
| T18H(C230C233) – intra-linked | 9.4 | 0.4 | 5.8 | 0.9 | N.D. | N.D. |
| T20H(C265)-T20H(C265) | 4.9 | 1.4 | 1.5 | 0.3 | N.D. | N.D. |
| T20H(C265)-T39H(C429) | 1.1 | 0.5 | 1.5 | 0.3 | N.D. | N.D. |
| T20H(C265)-T16L(C193) | 1.0 | 1.7 | N.D. | N.D. | N.D. | N.D. |
| T26H(C325)-T34H(C371) | 1.6 | 1.7 | 0.6 | 0.1 | N.D. | N.D. |
| T26H(C325)-T39H(C429) | 0.7 | 0.3 | 0.5 | N.D. | N.D. | N.D. |
| T26H(C325)-T16L(C193) | 0.2 | 0.8 | N.D. | N.D. | N.D. | N.D. |
The percent scrambled disulfide was obtained by the measured amount of scrambled disulfide divided by the total disulfide-linked forms.