Figure 3. Affect of a supplemental coding sequence of FCGRT on PCR ratios. (A) Results of real-time PCR with Hs02236230 (black bars) and Hs02001837 (gray bars). Ratio values are indicated above respective bars. (B) Southern blot analysis following HindIII digestion of corresponding DNA samples. * Bands of identical size corresponding to endogenous FCGRT. ** bands only present in FcRn Jurkat or ΔFcRn Jurkat DNA samples lane corresponding to FCGRT additional insertion.