Figure 6.

MiR-141 is upregulated in senescent cells. (A) Expression of miR-141 was studied by qRT-PCR in early passage (proliferating) and late passage (senescent) cells. (B) MRC5 cells were either mock treated or treated with premature senescence-inducing reagents H₂O₂ (100 µM for 4 h followed by 24 h growth in normal medium without H2O2) and sodium butyrate (NaB) (4 mM for 24 h followed by 24 h growth in normal medium without NaB), and expression of miR-141 was determined by qRT-PCR analysis. In each case induction of senescence was confirmed by staining for SA-β-Gal and EdU markers. Error bars represent ± SD *, P < 0.05.