Figure 2. Quantification of the fluorescence intensity of the reactivity of S. aureus CP5 strain Reynolds and CP8 strain MN8 to antibody to the homologous CP antigen and PNAG. Binding of primary rabbit antibody to purified CP5 or CP8 conjugate antigens to S. aureus cells detected with anti-rabbit IgG secondary antibody conjugated to AlexaFluor (AF) 588 (red). Human mAb F598 to PNAG directly conjugated to AF 488 (Green) was used to detect PNAG on the bacterial surface. Histograms depict analysis of the co-localization of red and green pixels in samples reacted with antibody to both CP and PNAG antigens across the distances, in microns (µm), depicted on the X-axis. Arrows on photomicrographs indicate regions analyzed.