Figure 1. Characterization of the purified rVP6 and dl2/6-VLPs. (A) Purity and integrity analysis of RV rVP6 (lane 1) and dl2/6-VLPs (lane 2) with SDS-PAGE followed by PageBlue staining. Lane M illustrates molecular weight marker. Corresponding molecular weights are indicated with arrows on the right of the gel image. (B) Electron microscopy images of morphological structures assembled by RV rVP6 (panel 1) and dl2/6-VLPs (panel 2) corresponding to the SDS-PAGE lanes 1 and 2 (A) respectively. Protein structures were examined after negative staining with 3% uranyl acetate pH 4.6. (C) Evaluation of antigenicity of the purified rVP6 and dl2/6-VLPs at different concentrations with ELISA for total IgG antibodies using human polyclonal anti-rotavirus serum. Mean OD values with standard errors of duplicate wells are shown.