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. 2014 Jan 21;9(1):e86137. doi: 10.1371/journal.pone.0086137

Figure 4. Secretome profile in the conditioned culture medium of ADHLSC and HSC.

Figure 4

The Elisa analyses were performed on supernatants (n = 3 including the diseased donor, analyzed at passage 5 and passage 7) collected 24 hours after incubation with serum free medium. A, the collagen secretion was evaluated by measuring the procollagen type-I C-Peptide, precursor of the collagen type I, by Elisa. No significant difference was observed between HSC and ADHLSC. B, TGFβ1 secretion; ADHLSC and HSC secrete an equivalent amount of TGFβ1 in the culture supernatant. C, HGF secretion; we observed a significant difference in HGF secretion between HSC and ADHLSC. The liver stem/progenitor cells seem to secrete HGF about three times more than HSC.