Table 4. Phenotypic characterization of ADHLSC and HSC by flow cytometry for mesenchymal stem cells, hematopoietic cells and extracellular matrix markers.
ADHLSC | HSC | ||||||
P5 | P11 | P5 | P11 | ||||
Mesenchymal stem cells markers | |||||||
ASMA | 87,8±4,7% | 83,8±2,9% | 91,9±5,5% | 87,4±12,6% | |||
CD73 | 88,2±5,9% | 90,2±6,1% | 89,9±2,5% | 82,8±20,1% | |||
CD90 | 97,2±2,7% | 92,7±7,8% | 95,6±2,3% | 88,7±10,4% | |||
Hematopoietic cells markers | |||||||
CD133 | 0,7±0,2% | 1,3±0,3% | 2,1±2,2% | 2,2±2,3% | |||
CD45 | 1,4±0,8% | 2,6±3,1% | 1,7±1,0% | 1,8±1,9% | |||
CD117 | 0±0% | 0,07±0,12% | 0,3±0,4% | 0,03±0,06% | |||
Extracellular matrix markers | |||||||
CD49b | 95,6±1,1% | 95,5±2% | 89,7±8,0% | 90±9,7% | |||
CD29 | 86,4±10,2% | 60,8±11% | 77±21,5% | 56,6±49,64% | |||
CD44 | 77,5±8,6% | 79,7±9,2% | 68±4,1% | 75±18,9% |
Data presented as mean percentage of immunopositive cells from four different donors at early (P5) and late(P11) passages. No significant difference was observed between the two cell populations.