Figure 1. HLA DR binding affinities for immunoinformatic-predicted H1N1 influenza cross-conserved epitopes. Peptide identifiers and sequences are noted in the first and second columns, respectively. IC₅₀ values in μM units were calculated from curves fitted to dose-dependence competition binding data for each peptide-HLA DR allele pair. Peptide binding affinity is shown according to the following classification: IC₅₀ < 1 µM (black), 1 µM < IC₅₀ < 10 µM (dark gray), 10 µM < IC₅₀ < 50 µM (gray), 50 µM < IC₅₀ < 100 µM (light gray), IC₅₀ > 100 µM (lightest gray). IC₅₀ values too high to accurately measure under binding conditions tested are considered non-binders (NB; shown in white cells).