Figure 5. Simultaneous vaccinations with Vaxfectin® adjuvanted SIV gag and env DNAs. (A) Outline of the DNA vaccination. (B-D) Reciprocal endpoint binding antibody titers for p27gag (B), SIVmac251 Env (C) and SIVmac239 Env (D) are shown in log. (E-H) Reciprocal endpoint neutralizing antibodies titers against the T cell line-adapted SIVmac251-TCLA measured in M7-Luc cells (E) and against the transmitted SIVmac251 clone 35014 (M766) (F) and heterologous SIVsmE660 Env CG7G (G) and CG7V (H) using the TZM-bl assay are shown in log. Arrows indicate env DNA vaccinations. Neutralization titers shown are the log of the reciprocal dilution of sample that reduced the signal by 50% compared with virus in the absence of sample.