Figure 2. Immunological testing of TcdA-specific hybridoma clones generated from TcdA-C DNA vaccine immunized mice. (A) ELISA of culture supernatants (1:100 dilution) from selected hybridoma clones against TcdA-C. (B) Western blot analysis of mAb purified from hybridoma culture supernatants against TcdA-C protein expressed in transiently transfected 293T cell supernatant (S) and cell lysate (L). Supernatant (S) and cell lysate (L) of 293T cells transfected by the empty vector were included as negative control. The TcdA-C specific mAb used for Western blot analysis was at 1 µg/ml.