Figure 8. Comparison of TcdA toxin binding activities for purified mAb 5D8 (A) or 2C7 (B) produced either from hybridoma culture or from supernatant of 293T cells co-transfected with paired Ig gamma and kappa chain clones. ELISA curves labeled with solid squares indicate the mAb produced by hybridoma and open circles indicate mAb produced by cotransfection of paired Ig heavy and kappa chain clones. TcdA toxin was used as coating antigen (1 µg/ml).