Table 1.
Colony-forming ability of various differentiation stages of HepaRG® cells in TK-NOG livers.
| Experiment No. | HepaRG® stage | Mouse ID No. | BW (g) | hAlb (μg/mL) | Area (cm2) | Hepatocyte-like colony |
Biliary-like colony |
||
|---|---|---|---|---|---|---|---|---|---|
| Number | Colonies/cm2 | Number | Colonies/cm2 | ||||||
| 1 | D35 | 2 | 15.2 | ND | 3.6 | 0 | 0 | 0 | 0 |
| 3 | 24.0 | ND | 4.3 | 6 | 1.4 | 5 | 1.2 | ||
| 4 | 26.5 | ND | 3.7 | 1 | 0.3 | 0 | 0 | ||
| 5 | 25.7 | ND | 4.0 | 0 | 0 | 1 | 0.3 | ||
| 8 | 27.0 | ND | 4.0 | 7 | 1.8 | 1 | 0.3 | ||
| 2 | D1 | 2 | 28.6 | ND | 3.3 | 0 | 0 | 0 | 0 |
| 3 | 28.0 | ND | 4.9 | 0 | 0 | 31 | 6.3 | ||
| D7 | 1 | 27.9 | 6.9 | 4.5 | 24 | 5.3 | 43 | 9.5 | |
| 5 | 28.9 | ND | 4.0 | 3 | 0.7 | 23 | 5.7 | ||
| D21 | 9 | 26.9 | 14.2 | 3.8 | 40 | 10.5 | 26 | 6.8 | |
| 14 | 26.5 | ND | 2.9 | 21 | 7.4 | 37 | 13.0 | ||
| D35 | 10 | 25.2 | ND | 2.8 | 2 | 0.7 | 1 | 0.4 | |
| 12 | 27.7 | ND | 3.0 | 4 | 1.3 | 4 | 1.3 | ||
D1, 7, 21 and 35 in HepaRG® stage indicate the number of days after seeding. BW: body weight. The hAlb level of each animal is shown. ND: not detected by ELISA. Area: observed cross-sections were measured and indicated as centimeter square (cm2). Colonies per centimeter square (colonies/cm2): number of colonies per area of observation.