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FRK1 gene expression in seedlings treated with flg22. After treatment of 10-d-old seedlings with 100 nM flg22 or water, quantitative RT-PCR experiments were performed on cDNA generated from three independent biological replicates to analyze FRK1 gene expression as described by Mosher, et al.17 Expression values were calculated as a ratio to the housekeeping gene EF1α and presented as fold induction. Bars represent mean ratios +/− SEM.
