Skip to main content
. 2014 Feb;184(2):376–381. doi: 10.1016/j.ajpath.2013.10.016

Figure 2.

Figure 2

Classical MC activators elicit degranulation and release of histamine and renin from mature BMMCs. BMMCs were incubated for 20 minutes at 37°C with A23187 (1 μmol/L), c48/80 (300 μg/mL), or ATP (1 mmol/L). A–C: Release of β-HEX (A), histamine (B), and renin protein (C). Percentages represent the percent ratio between supernatant and total β-HEX or histamine. D: Renin activity as affected by the three MC activators in the absence or presence of the renin inhibitor BILA (100 nmol/L) was measured in BMMC supernatant in terms of angiotensin I (Ang I) formation (μg/hour per mg of total protein). Data are expressed as means ± SEM. Number of experiments (left to right): n = 10, 7, 6, and 10 (A); n = 7, 14, 5, and 6 (B); n = 4, 5, 4, and 7 (C); and n = 6, 5, 8, 4, 3, 13, and 6 (D). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus control. †P < 0.01, ††P < 0.001 versus absence of BILA.