Table 1.
List of Antibodies Used in Immunofluorescence
| Antigen | Cell type | Source | Antibody type | Working dilution |
|---|---|---|---|---|
| TLR231,32 | NA | Abcam (Cambridge, MA) | Rb polyclonal | 1:50 |
| GFAP | Astrocyte | Sigma (St. Louis, MO) | Ms IgG1, labeled with Cy3 | 1:200 |
| S100 | Glial, ependymal | Sigma | Rb polyclonal | 2.5 μg used with zenon |
| IBA-1 | Microglia | Wako (Richmond, VA) | Rb polyclonal | 2.5 μg used with zenon |
| NeuN | Neuron | Chemicon (Billerica, MA) | Ms IgG1 | 1:10 |
| F4/80 | Microglia/macrophage | Santa Cruz (Dallas, TX) | Rat IgG1 | 1:10 |
| Glut-1 | Endothelial cells | Abcam | Ms IgG1 | 1:40 |
NA, not applicable.