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. 2014 Feb;184(2):541–555. doi: 10.1016/j.ajpath.2013.10.022

Figure 1.

Figure 1

Loss of Cav-1 results in BRB hyperpermeability. A: Cav-1 KO and WT mice were intravenously injected with 4-kDa FITC-dextran, and BRB permeability was quantified. Cav-1 KO retinas exhibit a two-fold increase in leakage. B and C: Endogenous albumin staining (green) of retinal flatmount from WT (B) and Cav-1 KO (C) mice reveals leakage site (arrow) in the Cav-1 KO mouse. D and E: FITC-conjugated albumin was injected into WT (D) and Cav-1 KO (E) mice; retinas were flat-mounted and visualized for albumin extravasation. Compared with WT, the Cav-1 KO retina exhibits more leakage (arrow), and over a larger area, around a vein. F and G: IHC of albumin (green) on frozen sections of WT (F) and Cav-1 KO retinas (G). Vessels were immunolabeled with anti–collagen IV antibody (red). Extravasation of albumin is seen around the large vessels in the Cav-1 KO retina (arrows), but not in the WT or in nonleaky vessels in the Cav-1 KO retina. Representative images are shown. Data are expressed as means ± SEM. n = 5. ∗∗P < 0.01 versus WT. Scale bar = 50 μm. Alb, albumin; Col IV, collagen IV.