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. 2014 Feb;184(2):541–555. doi: 10.1016/j.ajpath.2013.10.022

Figure 6.

Figure 6

Loss of Cav-1 results in venous enlargement independent of eNOS action. Retinal flatmounts were prepared from WT, Cav-1 KO, Cav-1 and eNOS DKO, and eNOS KO mice and were immunostained with anti-CD31 antibody to label the vasculature. A: Schematic representation of locations used for obtaining diameter measurements. B: Representative images of branch retinal veins showing enlarged vessels in mice in which Cav-1 is deleted (ie, Cav-1 KO and DKO mice). Scale bar = 50 μm. C: Quantification of venous enlargement shows the lack of eNOS effect on enlargement. D: Quantification of venous diameter in the L-NAME- and vehicle-treated WT and Cav-1 KO retinas shows that transient NOS inhibition does not correct venous enlargement. Data are expressed as means ± SEM. n = 13 (WT), n = 15 (Cav-1), n = 11 (DKO), and n = 7 (eNOS KO) (A). n = 4–7 per treatment group (B). ∗∗P < 0.01, ∗∗∗∗P < 0.0001 versus WT; †P < 0.05, †††P < 0.001 versus eNOS KO (C). ∗∗P < 0.01, ∗∗∗P < 0.001 versus WT PBS; †††P < 0.001, ††††P < 0.0001 versus WT L-NAME (D). Scale bar = 50 μm. One-way analysis of variance followed by Newman–Keuls post hoc test.