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. 2014 Feb;184(2):541–555. doi: 10.1016/j.ajpath.2013.10.022

Figure 8.

Figure 8

Loss of Cav-1 alters the mural cell phenotype in enlarged branch retinal vein proximal to the ONH. WT (A and B) and Cav-1 KO (C and D) retinas were immunostained with antibodies against CD31 (red) and NG2 (green). Boxed areas in A and C are enlarged as panels B and D, respectively. NG2+ cells on Cav-1 KO retinal veins have a spindly appearance (arrowhead) (D). These cells, compared with counterparts on WT retinal veins, had thinner processes (arrows) (B and D). NG2 staining on arteries seemed normal. Immunostaining of WT (E–H) and Cav-1 KO (I–L) retinas with antibodies against mural cell markers NG2 (green) (F and J), PDGFRβ (blue) (G and K), and αSMA (red) (H and L) confirmed the spindly appearance of venous smooth muscle cells on branch retinal veins immediately proximal to entering the ONH in Cav-1 KO retina (I and J), compared with WT retina (E and F). αSMA staining further supported the observed phenotypic changes in mural cell morphology in WT (H) and KO (L) retinas. Mural cell staining on arteries was similar in WT and Cav-1 KO retinas. E and I represent merged images. Scale bars: 10 μm (A, C, and E–L); 5 μm (B and D). a, artery; v, vein.