Table 1.
Primers used for bisulfite sequencing
| Gene | Primer sequence (5′–3′) | Size (bp) | Annealing temperature |
|---|---|---|---|
| H19 | F1:GAGTATTTAGGAGGTATAAGAATT | 423 | 45 °C |
| R1:ATCAAAAACTAACATAAACCCCT | |||
| F2:GTAAGGAGATTATGTTTATTTTTGG | |||
| R2:CCTCATTAATCCCATAACTAT | |||
| Peg3 | F1:TGATAATAGTAGTTTGATTGGTAGGG | 444 | 45 °C |
| R1:TAATTCACACCTAAAACCCTAAAACC | |||
| F2:TTTTGTAGAGGATTTTGATAAGGAGG | |||
| R2:AAATACCACTTTAAATCCCTATCACC | |||
| Rasgrf1 | F1:AAGATAGTTTAGATATGGAATTTTGG | 221 | 60 °C |
| R:ATAATACAACAACAACAATAACAATC | |||
| F2:GATTTTTTAGAGAGTTTATAAAGTTAG | |||
| Line1 | F1:GTTAGAGAATTTGATAGTTTTTGGAATAGG | 250 | 56 °C |
| R1:CCAAAACAAAACCTTTCTCAAACACTATAT | |||
| F2:TAGGAAATTAGTTTGAATAGGTGAGAGGT | |||
| R2:TCAAACACTATATTACTTTAACAATTCCCA |