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Primers used for RT-PCR
| Gene | Primer sequence (5′–3′) | Size (bp) | Annealing temperature |
|---|---|---|---|
| H19 | F: GTATGCCCTAACCGCTCAGTC | 136 | 57 °C |
| R: CCAACCTCCCTCCCTAGAAAC | |||
| Peg3 | F: TCAATGACCTCACAAGCCACCAG | 261 | 57 °C |
| R: CGGGCAACAGAGCGATGAAAGC | |||
| Gapdh | F: TCTTGGGCTACACTGAGGAC | 126 | 57 °C |
| R: CATACCAGGAAATGAGCTTGA |
