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. 2014 Jan 30;8:3. doi: 10.3389/fninf.2014.00003

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Copyright © 2014 Campagnola, Kratz and Manis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Screen capture of the Patch module after patching a neuron in a mouse cochlear nucleus brain slice. In this image, approximately 40 s have passed since the membrane was ruptured to begin whole-cell recording. This data was acquired using only an NI-6259 acquisition board and a MultiClamp 700A amplifier. (A) Plots showing the voltage clamp recording and command waveform. This data is used by the module to determine the membrane properties shown at center-left (input resistance, access resistance, etc.). (B) Plot showing history of input resistance over the last 80 s. Maximum seal resistance and time of break-in are both recorded in this data.