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. 2014 Jan 30;4:4. doi: 10.3389/fcimb.2014.00004

Figure 2.

Figure 2

HMGB1 correlates with severity of infection. Snap-frozen tissue biopsies (n = 11) from patients with erysipelas were immunohistochemically stained for S. pyogenes, neutrophils identified by neutrophil elastase positivity and HMGB1. (A) Correlation between neutrophil infiltration in relation to HMGB1 ACIA value (for details see Materials and Methods) in erysipelas biopsies. Significant correlation, as determined by Pearson's correlation test, is indicated by p- and r-values. (B) Image analysis data of HMGB1 in tissue from patients with erysipelas were compared with biopsies from patients with severe streptococcal soft tissue infection of clinical grade 1 and 2 (for details see Materials and Methods). Statistically significant differences were determined by 1way ANOVA, Kruskal-Wallis; p = 0.0023. (C) Representative immunohistochemical pictures of erysipelas tissue biopsies collected at the inflamed center, 5 cm outside the lesion and from the healthy leg. Bars indicate 50 μm. (D) Acquired computerized image analysis values of the whole tissue biopsy.