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. 2014 Jan 30;5:5. doi: 10.3389/fmicb.2014.00005

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Copyright © 2014 Costa, Oliveira, Sousa, Morais, Moreno, Monteiro-Moreira, Viegas and Vasconcelos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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JcTI-I purification. (A) Affinity-chromatography (Trypsin-Sepharose 4B column). Inset: SDS-PAGE: (1) molar mass markers; (2) crude extract; (3) 2.5% TCA fraction; (4) unbound fractions; (5) bound fractions. (B) Size exclusion chromatography (Sephacryl S-200 column) of JcTI-I purified after chromatography on Trypsin-Sepharose 4B column. Inset: SDS-PAGE: (1) molar mass markers; (2 and 3) JcTI-I (5 μg) in the absence and presence of 1% β-mercaptoethanol, respectively; (4) JcTI-I (5 μg) stained with Schiff’s reagent.