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. Author manuscript; available in PMC: 2015 Jan 20.
Published in final edited form as: Virology. 2013 Dec 8;449:207–214. doi: 10.1016/j.virol.2013.11.018

Figure 4. Comparison of TCV and R6A accumulation in Arabidopsis lines with altered levels of TIP gene expression.

Figure 4

(A) Diagram showing the construction of two transgenic lines of A. thaliana ecotype Col-0. asTIP was designed to reduce TIP expression in mature plants by RNA silencing from an estradiole inducible promoter. TIPup was designed to overexpress TIP constitutively under the control of the CaMV 35S promoter (E35S). (B) Semi-quantitative PCR evaluation of TIP transcript expression in wild type and TIP altered transgenic lines of Col-0. ACT2 was used as the endogenous control. PCR cycle number used is shown on the right side of the figure. (C) Northern blots showing accumulation of viral RNAs of wt TCV (T) or mutant R6A (R) in inoculated leaves (IL) at 2 dpi and in systemic leaves (SL) at 2, 4, 6, and 8 dpi developed as described in Fig. 1. The panels show accumulation in the asTIP line after estradiole treatment and in the constitutively up-regulated TIP line. These are representative panels of three experiment which yielded consistent results.